Evaluation of an adjuvanted hydrogel-based pDNA nanoparticulate vaccine for rabies prevention and immunocontraception.
Nanomedicine
; 21: 102049, 2019 10.
Article
em En
| MEDLINE
| ID: mdl-31279062
Immunocontraceptive vaccination is becoming an acceptable strategy in managing animal populations. Mass vaccination of dogs is the most cost-effective and efficient method to control rabies, and combination of rabies vaccination and animal population control will be an added advantage. In this study, we developed an adjuvanted hydrogel-based pDNA nanoparticulate vaccine for rabies protection and immunocontraception. In vivo, we observed an immune response skewed toward a Th2 type, in contrast to the Th1 type in our previous pDNA study. The observation was verified by the IgG2a/IgG1 ratio (<1), and cytokine expression profile of IL-4 and IFN-γ. The humoral immune response is key for rabies protection and a GnRH antibody-based immunocontraception. In mice, anti-GnRH antibody titers were detected 4â¯weeks after immunization and lasted for 12â¯weeks, post animal experiment was terminated. The adjuvanted pDNA nanoparticulate vaccine shows promise for future studies evaluating protection from rabies challenge and prevention of animal breeding.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Raiva
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Vacina Antirrábica
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Vacinas de DNA
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Imunidade Humoral
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article