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L-Alpha-glycerylphosphorylcholine can be cytoprotective or cytotoxic in neonatal rat cardiac myocytes: a double-edged sword phenomenon.
Tuboly, Eszter; Gáspár, Renáta; Ibor, Miguel Olias; Gömöri, Kamilla; Kiss, Bernadett; Strifler, Gerda; Hartmann, Petra; Ferdinandy, Péter; Bartekova, Monika; Boros, Mihály; Görbe, Anikó.
Afiliação
  • Tuboly E; Faculty of Medicine, Institute of Surgical Research, University of Szeged, Szeged, Hungary.
  • Gáspár R; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary.
  • Ibor MO; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary.
  • Gömöri K; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary.
  • Kiss B; Pharmahungary Group, Szeged, Hungary.
  • Strifler G; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Hartmann P; Faculty of Medicine, Institute of Surgical Research, University of Szeged, Szeged, Hungary.
  • Ferdinandy P; Faculty of Medicine, Institute of Surgical Research, University of Szeged, Szeged, Hungary.
  • Bartekova M; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Boros M; Pharmahungary Group, Szeged, Hungary.
  • Görbe A; Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia.
Mol Cell Biochem ; 460(1-2): 195-203, 2019 Oct.
Article em En | MEDLINE | ID: mdl-31280435
ABSTRACT
L-Alpha-glycerylphosphorylcholine (GPC) is a widely used food supplement. GPC has been shown to exert beneficial effects in several organs; however, the cardiac effects of GPC have yet to be investigated. The aim of the present study was therefore to map out the effects of GPC on cardiac myocytes, with or without ischemia-reperfusion insult. Neonatal rat cardiac myocytes were treated with GPC at 1, 10, 80, and 100 µM concentrations for 15 min, 3 h, or 24 h, respectively. Cell viability by calcein assay and the degree of oxidative stress by DHE (superoxide level) and H2DCF (total ROS accumulation) staining were measured. In separate experiments, cardiomyocytes were pre-treated with the optimal concentration of GPC for 3 h and then cells were exposed to 4 h of simulated ischemia followed by 2 h of reperfusion (SI/R). Cell viability was measured at the end of the SI/R protocol. In normoxic conditions, the 15-min and the 3-h GPC treatment did not affect cell viability, total ROS, and superoxide levels. Under SI/R conditions, the 3-h GPC treatment protected the cardiac myocytes from SI/R-induced cell death and did not alter the level of oxidative stress. The 24-h GPC treatment in normoxic conditions resulted in significant cell death and increased oxidative stress at each concentration. Here we provide the first evidence for the cytoprotective effect of short-term GPC treatment. However, long-term administration of GPC may exert cytotoxicity in a wide concentration range in cardiac myocytes. These results may draw attention to a comprehensive cardiac safety protocol for the testing of GPC.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Citoproteção / Miócitos Cardíacos / Glicerilfosforilcolina Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Citoproteção / Miócitos Cardíacos / Glicerilfosforilcolina Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article