Separation of Sialylated Glycan Isomers by Differential Mobility Spectrometry.
Anal Chem
; 91(15): 9916-9924, 2019 08 06.
Article
em En
| MEDLINE
| ID: mdl-31283185
ABSTRACT
Mass spectrometry has proven itself to be an important technology for characterizing intact glycoproteins, glycopeptides, and released glycans. However, these molecules often present significant challenges during analysis. For example, glycans of identical molecular weights can be present in many isomeric forms, with one form having dramatically more biological activity than the others. Discriminating among these isomeric forms using mass spectrometry alone can be daunting, which is why orthogonal techniques, such as ion mobility spectrometry, have been explored. Here, we demonstrate the use of differential mobility spectrometry (DMS) to separate isomeric glycans differing only in the linkages of sialic acid groups (e.g., α 2,3 versus α 2,6). This ability extends from a small trisaccharide species to larger biantennary systems and is driven, in part, by the role of intramolecular solvation of the charge site(s) on these ions within the DMS environment.
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1
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Espectrometria de Mobilidade Iônica
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article