A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count.

BACKGROUND AND OBJECTIVES: Rat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perform PRF based experiments on rats, a proper protocol of obtaining PRF from rats needs to be established. MATERIALS AND METHODS: 35 Wistar rats were used to obtain PRF by using cardiac puncture blood draw and quick subsequent centrifugation. The PRF samples wereanalyzed and compared to standard literature PRF composition. RESULTS: PRF samples analysis showed persistent results pertaining to known PRF composition. CONCLUSIONS: Our experiment has shown that our protocol of obtaining PRF is capable of providing high quality PRF from rats.