Downregulation of RPS15A by miR-29a-3p attenuates cell proliferation in colorectal carcinoma.
Biosci Biotechnol Biochem
; 83(11): 2057-2064, 2019 Nov.
Article
em En
| MEDLINE
| ID: mdl-31303129
ABSTRACT
miR-29a-3p has been reported to function as a tumor suppressor in several cancers. However, the biological function role of miR-29a-3p in colorectal carcinoma (CRC) has not been well investigated. In this study, we found that miR-29a-3p was at lower level expression in CRC tissues and cell lines. Experimental up-regulation miR-29a-3p with mimic could inhibit cell proliferation, but induced cell cycle arrest at G0/G1 phase and apoptosis in CRC cells. MiR-29a-3p overexpression significantly down-regulated the expression levels of CDK4, Cyclin D1, and Bax, but up-regulated the expression levels of p21 and Bcl-2 in DLD-1 cells. Moreover, ribosomal protein S15A (RPS15A) was predicted and confirmed as a direct target gene of miR-29a-3p. Furthermore, restoration of RPS15A could rescue the phenotypic changes caused by miR-29a-3p. The findings demonstrate miR-29a-3p inhibits CRC cell function possibly by targeting RPS15A, which might be exploited therapeutically in CRC.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Ribossômicas
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Neoplasias Colorretais
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Regulação para Baixo
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MicroRNAs
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article