Cell Cycle Perturbation Induces Collagen Production in Fibroblasts.
Int Heart J
; 60(4): 958-963, 2019 Jul 27.
Article
em En
| MEDLINE
| ID: mdl-31308330
ABSTRACT
Myocardial infarction (MI) occurs when the heart muscle is severely damaged due to a decrease in blood flow from the coronary arteries. During recovery from an MI, cardiac fibroblasts become activated and produce extracellular matrices, contributing to the wound healing process in the damaged heart. Inappropriate activation of the fibroblasts leads to excessive fibrosis in the heart. However, the molecular pathways by which cardiac fibroblasts are activated have not yet been fully elucidated.Here we show that serum deprivation, which recapitulates the cellular microenvironment of the MI area, strikingly induces collagen production in C3H/10T1/2 cells. Based on transcriptomic and pharmacological studies, we found that cell cycle perturbation is directly linked to collagen production in fibroblasts. Importantly, collagen synthesis is increased independently of the transcriptional levels of type I collagen genes. These results reveal a novel mode of fibroblast activation in the ischemic area, which will allow us to gain insights into the molecular mechanisms underlying cardiac fibrosis and establish a basis for anti-fibrotic therapy.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Colágeno
/
Miócitos Cardíacos
/
Infarto do Miocárdio
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article