High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes.
Nat Commun
; 10(1): 3120, 2019 07 16.
Article
em En
| MEDLINE
| ID: mdl-31311926
ABSTRACT
High-throughput single-cell RNA sequencing is a powerful technique but only generates short reads from one end of a cDNA template, limiting the reconstruction of highly diverse sequences such as antigen receptors. To overcome this limitation, we combined targeted capture and long-read sequencing of T-cell-receptor (TCR) and B-cell-receptor (BCR) mRNA transcripts with short-read transcriptome profiling of barcoded single-cell libraries generated by droplet-based partitioning. We show that Repertoire and Gene Expression by Sequencing (RAGE-Seq) can generate accurate full-length antigen receptor sequences at nucleotide resolution, infer B-cell clonal evolution and identify alternatively spliced BCR transcripts. We apply RAGE-Seq to 7138 cells sampled from the primary tumor and draining lymph node of a breast cancer patient to track transcriptome profiles of expanded lymphocyte clones across tissues. Our results demonstrate that RAGE-Seq is a powerful method for tracking the clonal evolution from large numbers of lymphocytes applicable to the study of immunity, autoimmunity and cancer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Análise de Célula Única
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Evolução Clonal
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article