Effect fingerprints of antipsychotic drugs on neural networks in vitro.
J Neural Transm (Vienna)
; 126(10): 1363-1371, 2019 10.
Article
em En
| MEDLINE
| ID: mdl-31321550
ABSTRACT
We compared the acute effect of typical (haloperidol) and atypical (aripiprazole, clozapine, olanzapine) antipsychotic drugs (APDs) on spontaneous electrophysiological activity of in vitro neuronal networks cultured on microelectrode arrays (MEAs). Network burst analysis revealed a "regularizing" effect of all APDs at therapeutic concentrations, i.e., an increase of network-wide temporal synchronization. At supratherapeutic concentrations, all APDs but olanzapine mediated a decrease of burst and spike rates, burst duration, number of spikes in bursts, and network synchrony. The rank order of potency of APDs was haloperidol > aripiprazole > clozapine > olanzapine (no suppression). Disruption of network function was not due to enhanced cell death as assessed by trypan blue staining. APDs promoted distinct concentration-dependent alterations yielding acute effect fingerprints of the tested compounds. These effects were rather characteristic for individual compounds than distinctive for typical vs. atypical APDs. Thus, this dichotomy may be of value in distinguishing clinical features but has no apparent basis on the network or local circuitry level.
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Base de dados:
MEDLINE
Assunto principal:
Antipsicóticos
/
Potenciais de Ação
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Córtex Cerebral
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Rede Nervosa
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article