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Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering.
Suzuki, Yuta; Kobayashi, Koya; Wakisaka, Yoshifumi; Deng, Dinghuan; Tanaka, Shunji; Huang, Chun-Jung; Lei, Cheng; Sun, Chia-Wei; Liu, Hanqin; Fujiwaki, Yasuhiro; Lee, Sangwook; Isozaki, Akihiro; Kasai, Yusuke; Hayakawa, Takeshi; Sakuma, Shinya; Arai, Fumihito; Koizumi, Kenichi; Tezuka, Hiroshi; Inaba, Mary; Hiraki, Kei; Ito, Takuro; Hase, Misa; Matsusaka, Satoshi; Shiba, Kiyotaka; Suga, Kanako; Nishikawa, Masako; Jona, Masahiro; Yatomi, Yutaka; Yalikun, Yaxiaer; Tanaka, Yo; Sugimura, Takeaki; Nitta, Nao; Goda, Keisuke; Ozeki, Yasuyuki.
Afiliação
  • Suzuki Y; Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.
  • Kobayashi K; Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.
  • Wakisaka Y; Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Deng D; Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.
  • Tanaka S; Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.
  • Huang CJ; Department of Photonics, National Chiao Tung University, 300 Hsinchu, Taiwan.
  • Lei C; Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Sun CW; Institute of Technological Sciences, Wuhan University, 430072 Wuhan, China.
  • Liu H; Department of Photonics, National Chiao Tung University, 300 Hsinchu, Taiwan.
  • Fujiwaki Y; Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.
  • Lee S; Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.
  • Isozaki A; Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Kasai Y; Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Hayakawa T; Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, 464-8601 Nagoya, Japan.
  • Sakuma S; Department of Precision Mechanics, Chuo University, 112-8551 Tokyo, Japan.
  • Arai F; Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, 464-8601 Nagoya, Japan.
  • Koizumi K; Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, 464-8601 Nagoya, Japan.
  • Tezuka H; Department of Creative Informatics, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Inaba M; Department of Creative Informatics, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Hiraki K; Department of Creative Informatics, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Ito T; Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Hase M; Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Matsusaka S; Japan Science and Technology Agency, 332-0012 Saitama, Japan.
  • Shiba K; Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Suga K; Department of Gastroenterology, Cancer Institute Hospital, Japanese Foundation for Cancer Research, 135-8550 Tokyo, Japan.
  • Nishikawa M; Clinical Research and Regional Innovation, Faculty of Medicine, University of Tsukuba, 305-8575 Ibaraki, Japan.
  • Jona M; Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, 135-8550 Tokyo, Japan.
  • Yatomi Y; Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, 135-8550 Tokyo, Japan.
  • Yalikun Y; Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Tanaka Y; Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Sugimura T; Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 113-0033 Tokyo, Japan.
  • Nitta N; Center for Biosystems Dynamics Research, RIKEN, 565-0871 Osaka, Japan.
  • Goda K; Center for Biosystems Dynamics Research, RIKEN, 565-0871 Osaka, Japan.
  • Ozeki Y; Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
Proc Natl Acad Sci U S A ; 116(32): 15842-15848, 2019 08 06.
Article em En | MEDLINE | ID: mdl-31324741
ABSTRACT
Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Imageamento Tridimensional / Citometria de Fluxo Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Imageamento Tridimensional / Citometria de Fluxo Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article