Activation by PHA of CD8 lymphocytes into clonal colony forming cells. Role of interleukin-1.
J Immunol Methods
; 110(2): 241-9, 1988 Jun 13.
Article
em En
| MEDLINE
| ID: mdl-3132508
ABSTRACT
Monoclonal T cell colonies can be grown in agar culture from quiescent T lymphocytes under PHA stimulation, provided that (1) a low number of T lymphocytes (less than or equal to 5 X 10(4)/ml) is seeded, (2) IL-2 is added to the culture, and (3) a high number of accessory B cells (greater than or equal to 5 X 10(5)/ml) is present in contact with the T lymphocytes. Under these culture conditions the colony progenitors can be ascribed to the CD4 subset, whereas CD8 lymphocytes do not generate colonies. This finding is surprising since both CD4 and CD8 lymphocytes may be cloned in liquid culture. We now report the appropriate conditions required to grow cytotoxic CD8 lymphocyte colonies in agar. CD8 colony growth is dependent upon IL-2-IL-2 receptor interaction and is inhibited by anti-IL-2 receptor antibodies. In addition to PHA, accessory B cells and IL-2, an additional signal provided by recombinant IL-1 is necessary for CD8 colony formation. Exogenous IL-1 can be replaced by irradiated CD4 lymphocytes which stimulate the expression of membrane IL-1 activity in the accessory B cells. In addition, colony growth from quiescent but not preactivated CD8 lymphocytes is inhibited by anti-IL-1 antibodies. Altogether, the data show that an IL-1 signal is required for the induction of IL-2 responsive IL-2 receptors on quiescent CD8 colony forming cells.
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Base de dados:
MEDLINE
Assunto principal:
Fito-Hemaglutininas
/
Células-Tronco Hematopoéticas
/
Ativação Linfocitária
/
Linfócitos T
/
Antígenos de Diferenciação de Linfócitos T
/
Interleucina-1
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article