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In vitro photodynamic inactivation effects of hypocrellin B on azole-sensitive and resistant Candida albicans.
Jan, Asif; Liu, Chengcheng; Deng, Hong; Li, Jiao; Ma, Wenpeng; Zeng, Xiaoyan; Ji, Yanhong.
Afiliação
  • Jan A; Department of Pathogenic Microbiology & Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 710061, PR China.
  • Liu C; Department of Pathogenic Microbiology & Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 710061, PR China. Electronic address: liuchengcheng@xjtu.edu.cn.
  • Deng H; Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, 21st North Street, Beijing, 100190, PR China. Electronic address: denghong010@iccas.ac.cn.
  • Li J; Department of Pathogenic Microbiology & Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 710061, PR China.
  • Ma W; Department of Pathogenic Microbiology & Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 710061, PR China.
  • Zeng X; The First Affiliated Hospital of College of Medicine, Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, PR China.
  • Ji Y; Department of Pathogenic Microbiology & Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 710061, PR China. Electronic address: jiyanhong@xjtu.edu.cn.
Photodiagnosis Photodyn Ther ; 27: 419-427, 2019 Sep.
Article em En | MEDLINE | ID: mdl-31325521
BACKGROUND AND AIM: The extensive use of antifungal drugs has led to resistance from Candida albicans. The search for alternative treatment against drug-resistant C. albicans is highly desirable. Antimicrobial photodynamic therapy (aPDT) is an emerging and promising approach for treating localized and superficial C. albicans infections. The aim of this study was to investigate the photodynamic inactivation (PDI) effects of hypocrellin B (HB) on azole-sensitive and resistant C. albicans in vitro. METHODS: The PDI efficacies of HB on standard C. albicans strain (ATCC 10231), azole-sensitive clinical isolate of C. albicans, and azole-resistant clinical isolate of C. albicans were assessed. The uptake of HB in C. albicans cells was investigated by confocal laser scanning microscopy (CLSM). The PDI effects on cellular structure and surface characteristics were investigated by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). RESULTS: HB exhibited no significant dark toxicity, but inactivated the azole-sensitive and resistant C. albicans in a light-dose and PS concentration-dependent manner. CLSM images indicated that PDI treated C. albicans cells showed stronger fluorescence compared to untreated cells. TEM images suggested that significant damage to the cell wall, membrane, and cytoplasm were induced by HB-mediated PDI. SEM analysis revealed that the surface of C. albicans cells became twisted and ruptured after PDI treatment. CONCLUSIONS: Azole-sensitive and resistant C. albicans could be effectively inactivated by HB in the presence of light, and HB-mediated aPDT shows promise as an antifungal treatment for C. albicans.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Perileno / Fotoquimioterapia / Quinonas / Candida albicans Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Perileno / Fotoquimioterapia / Quinonas / Candida albicans Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article