CRISPR-Cas bioinformatics.
Methods
; 172: 3-11, 2020 02 01.
Article
em En
| MEDLINE
| ID: mdl-31326596
ABSTRACT
Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins (Cas) are essential genetic elements in many archaeal and bacterial genomes, playing a key role in a prokaryote adaptive immune system against invasive foreign elements. In recent years, the CRISPR-Cas system has also been engineered to facilitate target gene editing in eukaryotic genomes. Bioinformatics played an essential role in the detection and analysis of CRISPR systems and here we review the bioinformatics-based efforts that pushed the field of CRISPR-Cas research further. We discuss the bioinformatics tools that have been published over the last few years and, finally, present the most popular tools for the design of CRISPR-Cas9 guides.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biologia Computacional
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Sistemas CRISPR-Cas
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Edição de Genes
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article