Micronucleus assay in human fibroblasts: a measure of spontaneous chromosomal instability and mutagen hypersensitivity.
Environ Mol Mutagen
; 12(1): 3-13, 1988.
Article
em En
| MEDLINE
| ID: mdl-3133207
ABSTRACT
By comparing fibroblast strains derived from individuals exhibiting chromosome instability and/or mutagen hypersensitivity (Cockayne syndrome, ataxia telangiectasia, and Fanconi anemia) with strains derived from healthy donors, the fibroblast micronucleus assay has been established as a reproducible measure of the genotypic variation in spontaneous or mitomycin C (MMC)-induced chromosomal instability. The patient strains that were moderately or exquisitely sensitive to MMC, whereas the mildly sensitive strain (Cockayne syndrome) overlapped with the control range. The reproducibility of the assay was evaluated within and between experiments. Paired comparison analyses between duplicate cultures and between repeat experiments failed to show any significant differences between micronucleus frequencies within strains, whereas a significant differences in the spontaneous micronucleus frequencies between strains was observed. In addition to its value as a test system for genotoxins, the fibroblast micronucleus assay may be useful for investigating genetically determined hypersensitivity to mutagens, elevated spontaneous chromosomal breakage, and chromosome segregation errors.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Ataxia Telangiectasia
/
Núcleo Celular
/
Síndrome de Cockayne
/
Nanismo
/
Anemia de Fanconi
/
Fibroblastos
/
Anemia Aplástica
/
Mitomicinas
/
Testes de Mutagenicidade
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article