Purification and characterisation of two forms of toxin B produced by Clostridium difficile.
FEBS Lett
; 233(2): 417-20, 1988 Jun 20.
Article
em En
| MEDLINE
| ID: mdl-3133248
ABSTRACT
Toxin B from Clostridium difficile was purified to homogeneity by gel filtration and high resolution ion exchange chromatography. Two forms of toxin B were found. Form 1 which seemed to consist of two identical subunits of 220-300 kDa; femtogram amounts of this toxin induced rounding of fibroblast cells. Form 2 contained subunits of 43 kDa and 105 kDa; the stoichiometric ratio probably being 41; picogram amounts were needed to induce rounding of fibroblast cells. Immunological studies suggested that both subunit types were antigenic and had epitopes which were identical with those of form 1.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Toxinas Bacterianas
/
Clostridium
Limite:
Humans
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article