The determination of patulin from food samples using dual-dummy molecularly imprinted solid-phase extraction coupled with LC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1125: 121714, 2019 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-31357106
ABSTRACT
A molecularly imprinted polymer (MIP) with specific adsorption for patulin was successfully polymerized by precipitation polymerization using 2-oxindole (2-oxin) and 6-hydroxynicotinic acid (6-HNA) as dummy template molecules, methylacrylic acid (MAA) as a functional monomer, trimethylolpropane trimethacrylate (TRIM) as a crosslinker, 2,2-azobis-(2-methylpropionitrile) (AIBN) as a initiator, and methanol as a porogen solvent. The molecularly imprinted solid phase extraction (MI-SPE) column was prepared using the polymer as a sorbent and applied for the selective extraction of patulin from real samples. The results showed that the MI-SPE method had high selectivity and specific adsorption towards patulin with mean recoveries ranged between 81.3% and 106.3% and a relative standard deviation (RSD) < 4.5%. Additionally, the developed MI-SPE method coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) exhibited good linearity in the range of 1-100â¯ngâ¯mL-1 with correlation coefficients (R2) >0.998. The limits of detection (LODs, S/Nâ¯=â¯3) were 0.05-0.2â¯ngâ¯g-1, and the limits of quantification (LOQs, S/Nâ¯=â¯10) were 0.2-0.5â¯ngâ¯g-1. The developed method showed a better purification and higher patulin recovery for real samples than the quick, easy, cheap, effective, rugged, safe "QuEChERS" method.
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Base de dados:
MEDLINE
Assunto principal:
Patulina
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Contaminação de Alimentos
/
Cromatografia Líquida de Alta Pressão
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Espectrometria de Massas em Tandem
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Extração em Fase Sólida
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article