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Quick electrochemical immunoassay for hantavirus detection based on biochar platform.
Martins, Gustavo; Gogola, Jeferson L; Caetano, Fabio R; Kalinke, Cristiane; Jorge, Taíssa R; Santos, Claudia N D; Bergamini, Márcio F; Marcolino-Junior, Luiz H.
Afiliação
  • Martins G; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil.
  • Gogola JL; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil.
  • Caetano FR; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil.
  • Kalinke C; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil.
  • Jorge TR; Instituto Carlos Chagas, FIOCRUZ, CEP 81310-020, Curitiba, PR, Brazil.
  • Santos CND; Instituto Carlos Chagas, FIOCRUZ, CEP 81310-020, Curitiba, PR, Brazil.
  • Bergamini MF; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil.
  • Marcolino-Junior LH; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil. Electronic address: luiz1berto@ufpr.br.
Talanta ; 204: 163-171, 2019 Nov 01.
Article em En | MEDLINE | ID: mdl-31357278
This work describes the first method using biochar (BC) as carbonaceous platform for immunoassay application. BC is a highly functionalized material obtained through biomass pyrolysis under controlled conditions. Due to the highly functionalized surface, covalent binding between BC and biomolecules can be performed by EDC/NHS conjugation. The application of the modified electrode was done with Hantavirus, that are etiologic agents mainly transmitted by wild rodents. Among its pathologies Hantavirus Cardiopulmonary Syndrome (HCPS) arises at Americas, caused by Hantavirus Araucária and reaches 40% lethality. The diagnostic is based on the presence of specific hantavirus nucleoprotein (Np), under viremic condition or IgG2b antibodies (Ab), during first symptoms. The results presented a device sensitivity of 5.28 µA dec-1 and a LOD of 0.14 ng mL-1 to the Np detection, ranging from 5.0 ng mL-1 to 1.0 µg mL-1, the Ab detection works as qualitative type sensor above 200 ng mL-1. Both sensors were evaluated its selectivity and serum samples; selectivity against Gumboro disease, VP2 protein, and antibody IgG2a against Yellow fever disease (YF), respectively. So, the devices here proposed are promising tool suitable for both rodent and human hantavirus clinical surveys.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carvão Vegetal / Orthohantavírus Tipo de estudo: Diagnostic_studies / Qualitative_research Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carvão Vegetal / Orthohantavírus Tipo de estudo: Diagnostic_studies / Qualitative_research Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article