Study of the Inducible Cross-Linking Reaction to mRNA and the Effect on the Translation.
Chem Pharm Bull (Tokyo)
; 67(8): 877-883, 2019.
Article
em En
| MEDLINE
| ID: mdl-31366836
ABSTRACT
The 4-vinylpyrimidin-2-one nucleoside (T-vinyl) forms a cross-link with the RNA containing uracil at the complementary site at a high reaction rate. To obtain the stable T-vinyl derivative so that its reactivity is protected until it access to the target site, several derivatives were investigated, and the 2-thiopyridinyl- and 2-thiopyrimidinyl T-vinyl derivatives were determined to be good candidates. The 2-thiopyrimidinyl T-vinyl derivative was found to more efficiently cross-link with mRNA albeit having a better stability than the 2-thiopyridinyl T-vinyl derivative. The investigation using the luciferase (Luc) mRNA, the synthetic mRNA and non-cellular translation system revealed that the translation is terminated at the end of the cross-linked duplex between the mRNA and the oligoribonucleotide (ORN). Thus, the 2-thiopyrimidinyl T-vinyl derivative has successfully demonstrated both a good stability and high efficiency for the cross-linking reaction, and expanded its applicability in biological applications.
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Base de dados:
MEDLINE
Assunto principal:
Oligorribonucleotídeos
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Compostos de Vinila
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RNA Mensageiro
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Reagentes de Ligações Cruzadas
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Nucleosídeos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article