Enzymatic Biotransformation of Balloon Flower Root Saponins into Bioactive Platycodin D by Deglucosylation with Caldicellulosiruptor bescii ß-Glucosidase.
Int J Mol Sci
; 20(16)2019 Aug 07.
Article
em En
| MEDLINE
| ID: mdl-31394870
ABSTRACT
Platycodin D (PD), a major saponin (platycoside) in Platycodi radix (balloon flower root), has higher pharmacological activity than the other major platycosides; however, its content in the plant root is only approximately 10% (w/w) and the productivities of PD by several enzymes are still too low for industrial applications. To rapidly increase the total PD content, the ß-glucosidase from Caldicellulosiruptor bescii was used for the deglucosylation of the PD precursors platycoside E (PE) and platycodin D3 (PD3) in the root extract into PD. Under the optimized reaction conditions, the enzyme completely converted the PD precursors into PD with the highest productivity reported so far, increasing the total PD content to 48% (w/w). In the biotransformation process, the platycosides in Platycodi radix were hydrolyzed by four pathways deapiosylated (deapi)-PE â deapi-PD3 â deapi-PD, PE â PD3 â PD, polygalacin D3 â polygalacin D, and 3â³-O-acetyl polygalacin D3 â 3â³-O-acetyl polygalacin D.
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Base de dados:
MEDLINE
Assunto principal:
Saponinas
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Triterpenos
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Biotransformação
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Beta-Glucosidase
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Raízes de Plantas
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Platycodon
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Firmicutes
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article