Expanding C-T base editing toolkit with diversified cytidine deaminases.
Nat Commun
; 10(1): 3612, 2019 08 09.
Article
em En
| MEDLINE
| ID: mdl-31399578
ABSTRACT
Base editing tools for cytosine to thymine (C-T) conversion enable genome manipulation at single base-pair resolution with high efficiency. Available base editors (BEs) for C-T conversion (CBEs) have restricted editing scopes and nonnegligible off-target effects, which limit their applications. Here, by screening diversified lamprey cytidine deaminases, we establish various CBEs with expanded and diversified editing scopes, which could be further refined by various fusing strategies, fusing at either N-terminus or C-terminus of nCas9. Furthermore, off-target analysis reveals that several CBEs display improved fidelity. Our study expands the toolkits for C-T conversion, serves as guidance for appropriate choice and offers a framework for benchmarking future improvement of base editing tools.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Timina
/
Citidina Desaminase
/
Citosina
/
Edição de Genes
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article