Dual-readout immunosensor constructed based on brilliant photoelectrochemical and photothermal effect of polymer dots for sensitive detection of sialic acid.
Biosens Bioelectron
; 142: 111567, 2019 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-31400731
ABSTRACT
A delicate dual-readout immunosensor based on tetraphenylporphyrin-polymer dots (TPP-Pdots) with brilliant photoelectrochemical and photothermal performance was first successfully fabricated for the ultrasensitive detection of sialic acid (SA). Herein, TPP-Pdots with good biocompatibility, extraordinary light-harvesting ability and excellent photothermal conversion efficiency was used to capture SA antibody as dual-functional bioprobe for generating photocurrent and temperature signal. Furthermore, the large surface and morphology-mediated of rutile-TiO2 (R-TiO2) was beneficial to load amounts of TPP-Pdots for improved PEC signal and photothermal signal. Importantly, the temperature readout resulted from the variation of target concentration could be easily obtained by a universal thermometer which was time-saving and cost-saving. Under the optimized experimental conditions, the photocurrent densities and temperature changes proportionally increased with the increasing of SA concentrations from 3.5â¯×â¯10-5â¯ng/mL to 35â¯ng/mL (Râ¯=â¯0.996). Impressively, the dual-readout approach proposed here not only featured with good accuracy and high sensitivity for SA detection, but also paved the way for the development of a dual-readout immunoassay based on PEC biosensor.
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Base de dados:
MEDLINE
Assunto principal:
Porfirinas
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Técnicas Biossensoriais
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Ácido N-Acetilneuramínico
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Nanopartículas
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article