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Could quantitative real-time polymerase chain reaction assay serve as an alternative test method to evaluate human epidermal growth factor receptor 2 status of gastric carcinoma in the South Asian setting?
Kannangara, D K S; Lokuhetty, M D S; Subasinghe, D; Gunawardene, Y I N S; Dassanayake, R S.
Afiliação
  • Kannangara DKS; Post Graduate Institute of Medicine, University of Colombo, Colombo, Sri Lanka.
  • Lokuhetty MDS; Department of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.
  • Subasinghe D; Department of Surgery, Faculty of Medicine, The National Hospital of Sri Lanka, University of Colombo/University Surgical Unit, Colombo, Sri Lanka.
  • Gunawardene YINS; Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Colombo, Sri Lanka.
  • Dassanayake RS; Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka. rsdassanayake@chem.cmb.ac.lk.
Indian J Gastroenterol ; 38(4): 317-324, 2019 08.
Article em En | MEDLINE | ID: mdl-31401730
BACKGROUND: Human epidermal growth factor receptor 2 (HER2) protein overexpression and/or HER2 gene amplification are/is linked to a dismal outcome of gastric carcinoma (GCa). Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) are key methods to identify patients for HER2 targeted therapy. Drawbacks of both the methods warrant novel tests. Hence, we evaluated the value of quantitative real-time polymerase chain reaction (qPCR) as an alternative test method, relative to IHC to detect HER2 status of GCa and to find relationship between these  results with demographic/clinicopathological data. METHOD: Twenty GCa patients with known IHC HER2 scores were evaluated. qPCR was performed for the HER2 gene and amyloid precursor protein (reference gene) in formalin-fixed paraffin-embedded GCa tissue. Cycle threshold values (Ct) were analyzed using the Pfaffl method to detect HER2 gene amplification. RESULTS: HER2 positivity rates by IHC and qPCR were 20% and 35%, respectively. The sensitivity and specificity of qPCR were 67% and 76%, respectively, relative to IHC. qPCR results were reproducible. The diagnostic consistency between IHC and qPCR (κ = 0.146) was slightly agreeable (0.01 < k < 0.20), with a 65% concordance. Based on McNemar's test, there was no significant difference between the results of the two tests. IHC HER2 protein expression had relationship with the tumor (TNM) stage and Lauren histological type (p < 0.05). Positive HER2 gene expression by qPCR showed relationship with depth of invasion, lymph node involvement, and degree of differentiation (p < 0.05). CONCLUSION: Cost-effective qPCR could serve as an alternative test method for detection of HER2 status of GCa. Both HER2 overexpression by IHC and gene amplification by qPCR are associated with adverse clinicopathological features.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Imuno-Histoquímica / Carcinoma / Receptor ErbB-2 / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male País como assunto: Asia Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Imuno-Histoquímica / Carcinoma / Receptor ErbB-2 / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male País como assunto: Asia Idioma: En Ano de publicação: 2019 Tipo de documento: Article