Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state.

Menges, Julia; Cremanns, Martina; Steenpass, Laura

Menges, Julia; Cremanns, Martina; Steenpass, Laura.

Afiliação

Menges J; Institute of Human Genetics, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, Germany.
Cremanns M; Institute of Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, Germany; Department of Medical Microbiology, Ruhr-University Bochum, Universitätsstraße 150, 44801 Bochum, Germany.
Steenpass L; Institute of Human Genetics, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, Germany. Electronic address: laura.steenpass@uni-due.de.

Stem Cell Res ; 39: 101517, 2019 08.

Article em En | MEDLINE | ID: mdl-31404748

ABSTRACT

ABSTRACT

Biallelic inactivation of the retinoblastoma tumor suppressor gene (RB1) causes formation of retinoblastoma, a retinal eye tumor occurring in early childhood. Using the CRISPR/Cas9 nickase system, exon 1 of RB1 was deleted, including the RB1 promoter. As a result, sublines were generated carrying deletions of RB1 exon 1/promoter on one or both alleles.

Assuntos

Éxons/genética; Regiões Promotoras Genéticas/genética; Retinoblastoma/genética; Alelos; Sistemas CRISPR-Cas/genética; Imunofluorescência; Heterozigoto; Humanos; Cariotipagem; Masculino; Repetições de Microssatélites/genética; Proteínas de Ligação a Retinoblastoma/genética; Ubiquitina-Proteína Ligases/genética

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Retinoblastoma / Éxons / Regiões Promotoras Genéticas Limite: Humans / Male Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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