Alkyne-Tagged PLGA Allows Direct Visualization of Nanoparticles In Vitro and Ex Vivo by Stimulated Raman Scattering Microscopy.
Biomacromolecules
; 20(10): 4008-4014, 2019 10 14.
Article
em En
| MEDLINE
| ID: mdl-31408325
ABSTRACT
Polymeric nanoparticles (NPs) are attractive candidates for the controlled and targeted delivery of therapeutics in vitro and in vivo. However, detailed understanding of the uptake, location, and ultimate cellular fate of the NPs is necessary to satisfy safety concerns, which is difficult because of the nanoscale size of these carriers. In this work, we show how small chemical labels can be appended to poly(lactic acid-co-glycolic acid) (PLGA) to synthesize NPs that can then be imaged by stimulated Raman scattering microscopy, a vibrational imaging technique that can elucidate bond-specific information in biological environments, such as the identification of alkyne signatures in modified PLGA terpolymers. We show that both deuterium and alkyne labeled NPs can be imaged within primary rat microglia, and the alkyne NPs can also be imaged in ex vivo cortical mouse brain tissue. Immunohistochemical analysis confirms that the NPs localize in microglia in the mouse brain tissue, demonstrating that these NPs have the potential to deliver therapeutics selectively to microglia.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Portadores de Fármacos
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Alcinos
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Nanopartículas
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Copolímero de Ácido Poliláctico e Ácido Poliglicólico
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article