Whole cell biosynthetic activity of Komagataella phaffii (Pichia pastoris) GS115 strains engineered with transgenes encoding Chromobacterium violaceum &#969;-transaminase alone or combined with native transketolase.

Henríquez, Maria-José; Braun-Galleani, Stephanie; Nesbeth, Darren N

Whole cell biosynthetic activity of Komagataella phaffii (Pichia pastoris) GS115 strains engineered with transgenes encoding Chromobacterium violaceum ω-transaminase alone or combined with native transketolase.

Henríquez, Maria-José; Braun-Galleani, Stephanie; Nesbeth, Darren N.

Afiliação

Henríquez MJ; Department of Biochemical Engineering, University College London, London, UK.
Braun-Galleani S; Department of Biochemical Engineering, University College London, London, UK.
Nesbeth DN; Department of Biochemical Engineering, University College London, London, UK.

Biotechnol Prog ; 36(1): e2893, 2020 01.

Article em En | MEDLINE | ID: mdl-31425642

Assuntos

Amino Álcoois/metabolismo; Chromobacterium/enzimologia; Escherichia coli/metabolismo; Engenharia de Proteínas; Saccharomycetales/metabolismo; Transcetolase/metabolismo; Amino Álcoois/química; Biotransformação; Escherichia coli/citologia; Transgenes

Palavras-chave

Komagataella phaffii; Pichia pastoris; biocatalyst; chiral amino-alcohol; transaminase; transketolase; whole-cell biocatalysis

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transcetolase / Engenharia de Proteínas / Chromobacterium / Saccharomycetales / Escherichia coli / Amino Álcoois Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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