Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking.
Bioconjug Chem
; 30(9): 2312-2316, 2019 09 18.
Article
em En
| MEDLINE
| ID: mdl-31433175
ABSTRACT
Mitochondria are essential targets for treatment of diseases with mitochondrial disorders such as diabetes, cancer, and cardiovascular and neurodegenerative diseases. Mitochondria penetrating peptides (MPPs) are composed of cationic and hydrophobic amino acids that can target and permeate the mitochondrial membrane. Herein, a novel d-argine-phenylalanine-d-argine-phenylalanine-d-argine-phenylalanine-NH2 (rFrFrF) was tagged with a rhodamine-based fluorescent chromophore (TAMRA). This probe (TAMRA-rFrFrF) exhibited advantageous properties for long-term mitochondria tracking as demonstrated by fluorescence microscopy. Cell viability assays and oxygen consumption rates indicate low cytotoxicity and high biocompatibility of the new contrast agent. Colocalization studies suggest that TAMRA-rFrFrF is a promising candidate for continuous mitochondrial tracking for up to 3 days.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Rodaminas
/
Peptídeos Penetradores de Células
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Rastreamento de Células
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Corantes Fluorescentes
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Mitocôndrias
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article