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miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and Survival.
Gagnon, John D; Kageyama, Robin; Shehata, Hesham M; Fassett, Marlys S; Mar, Darryl J; Wigton, Eric J; Johansson, Kristina; Litterman, Adam J; Odorizzi, Pamela; Simeonov, Dimitre; Laidlaw, Brian J; Panduro, Marisella; Patel, Sana; Jeker, Lukas T; Feeney, Margaret E; McManus, Michael T; Marson, Alexander; Matloubian, Mehrdad; Sanjabi, Shomyseh; Ansel, K Mark.
Afiliação
  • Gagnon JD; Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Sandler Asthma Basic Research Center, University of California, San Francisco
  • Kageyama R; Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Sandler Asthma Basic Research Center, University of California, San Francisco
  • Shehata HM; Virology and Immunology, Gladstone Institutes, San Francisco, CA 94158, USA.
  • Fassett MS; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Dermatology, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Mar DJ; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Sandler Asthma Basic Research Center, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Wigton EJ; Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Sandler Asthma Basic Research Center, University of California, San Francisco
  • Johansson K; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Sandler Asthma Basic Research Center, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Litterman AJ; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Sandler Asthma Basic Research Center, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Odorizzi P; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Simeonov D; Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Diabetes Center, University of California, San Francisco, San Francisco, CA 9
  • Laidlaw BJ; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Panduro M; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Sandler Asthma Basic Research Center, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Patel S; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Sandler Asthma Basic Research Center, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Jeker LT; Diabetes Center and Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Pathology, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Feeney ME; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • McManus MT; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Marson A; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Matloubian M; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Sanjabi S; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Virology and Immunology, Gladstone Institutes, San Francisco, CA 94158, USA.
  • Ansel KM; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA; Sandler Asthma Basic Research Center, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: mark.ansel@ucsf.edu.
Cell Rep ; 28(8): 2169-2181.e4, 2019 08 20.
Article em En | MEDLINE | ID: mdl-31433990
ABSTRACT
Coordinate control of T cell proliferation, survival, and differentiation are essential for host protection from pathogens and cancer. Long-lived memory cells, whose precursors are formed during the initial immunological insult, provide protection from future encounters, and their generation is the goal of many vaccination strategies. microRNAs (miRNAs) are key nodes in regulatory networks that shape effective T cell responses through the fine-tuning of thousands of genes. Here, using compound conditional mutant mice to eliminate miR-15/16 family miRNAs in T cells, we show that miR-15/16 restrict T cell cycle, survival, and memory T cell differentiation. High throughput sequencing of RNA isolated by cross-linking immunoprecipitation of AGO2 combined with gene expression analysis in miR-15/16-deficient T cells indicates that these effects are mediated through the direct inhibition of an extensive network of target genes within pathways critical to cell cycle, survival, and memory.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linfócitos T / Ciclo Celular / Diferenciação Celular / MicroRNAs / Memória Imunológica Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linfócitos T / Ciclo Celular / Diferenciação Celular / MicroRNAs / Memória Imunológica Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article