Transmission-mode MALDI-2 mass spectrometry imaging of cells and tissues at subcellular resolution.
Nat Methods
; 16(9): 925-931, 2019 09.
Article
em En
| MEDLINE
| ID: mdl-31451764
ABSTRACT
Matrix-assisted laser desorption-ionization mass spectrometry imaging in transmission-mode geometry (t-MALDI-MSI) can provide molecular information with a pixel size of 1 µm and smaller, which makes this label-free method highly interesting for characterizing the chemical composition of tissues and cells on a (sub)cellular level. However, a major hindrance for wider use of the technology is the reduced ion abundance at small pixel sizes. Here we mitigate this problem by use of laser-induced post-ionization (MALDI-2) and by adapting a t-MALDI-2 ion source to an Orbitrap mass analyzer. We demonstrate the crucial sensitivity and accuracy boosts that are achieved with this combination by visualizing the distribution of numerous phospho- and glycolipids in mouse cerebellum and kidney slices, and in cultured Vero B4 cells. With brain tissue, a pixel size of 600 nm was achieved. Our method could constitute a valuable new tool for research in cell biology and biomedicine.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
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Encéfalo
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Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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Células Epiteliais
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Imagem Molecular
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Rim
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article