Diverse strains of Actinobacillus lignieresii isolated from clinically affected cattle in a geographically restricted area.
Aust Vet J
; 97(11): 440-446, 2019 Nov.
Article
em En
| MEDLINE
| ID: mdl-31475335
ABSTRACT
OBJECTIVE:
To investigate whether an outbreak of Actinobacillus lignieresii was caused by one or multiple strains.METHODS:
Nine isolates of A. lignieresii were obtained from the lymph nodes of 15 affected cattle from two farms to determine whether a single strain was involved. An enterobacterial repetitive insertion consensus sequence (ERIC) PCR was used for genotyping, and the repeats-in-toxin genes were analysed by PCR and sequencing.RESULTS:
Isolates from the two farms belonged to two and three genotypes, with a total of four genotypes detected. Genes of the apxICABD operons of some strains had deletions in the apxIA (~697 bp) and in the apxID (~187 bp) genes. The toxin gene deletions and the ERIC PCR patterns suggested the involvement of different A. lignieresii genotypes.CONCLUSION:
There was no evidence that a unique genotype was associated with actinobacillosis on the two farms, confirming that this disease was associated with other contributing factors.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções por Actinobacillus
/
Doenças dos Bovinos
/
Actinobacillus
Limite:
Animals
País como assunto:
Oceania
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article