Monitoring the Formation of Amyloid Oligomers Using Photoluminescence Anisotropy.
J Am Chem Soc
; 141(39): 15605-15610, 2019 10 02.
Article
em En
| MEDLINE
| ID: mdl-31536338
ABSTRACT
The formation of oligomeric soluble aggregates is related to the toxicity of amyloid peptides and proteins. In this manuscript, we report the use of a ruthenium polypyridyl complex ([Ru(bpy)2(dpqp)]2+) to track the formation of amyloid oligomers at different times using photoluminescence anisotropy. This technique is sensitive to the rotational correlation time of the molecule under study, which is consequently related to the size of the molecule. [Ru(bpy)2(dpqp)]2+ presents anisotropy values of zero when free in solution (due to its rapid rotation and long lifetime) but larger values as the size and concentration of amyloid-ß (Aß) oligomers increase. Our assays show that Aß forms oligomers immediately after the assay is started, reaching a steady state at â¼48 h. SDS-PAGE, DLS, and TEM were used to confirm and characterize the formation of oligomers. Our experiments show that the rate of formation for Aß oligomers is temperature dependent, with faster rates as the temperature of the assay is increased. The probe was also effective in monitoring the formation of α-synuclein oligomers at different times.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Amiloide
/
Medições Luminescentes
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article