Detection and prognostic relevance of circulating tumour cells (CTCs) in Asian breast cancers using a label-free microfluidic platform.
PLoS One
; 14(9): e0221305, 2019.
Article
em En
| MEDLINE
| ID: mdl-31553731
ABSTRACT
OBJECTIVES:
We aimed to study the prevalence of CTCs in breast cancer (BC) patients undergoing neoadjuvant or palliative therapy with a label-free microfluidic platform (ClearCell FX), and its prognostic relevance in metastatic BC (mBC). MATERIALS ANDMETHODS:
Peripheral blood samples were collected from 108 BC patients before starting a new line of treatment ("baseline"), majority of whom had mBC (76/108; 70.4%). CTCs were retrieved by dean flow fractionation that enriched for larger cells, and enumerated using immunofluorescence-based staining. Progression-free survival (PFS) in mBC patients was analysed using Kaplan-Meier method; cox proportional hazard models were used for univariable and multivariable analyses.RESULTS:
The detection rate of CTCs before starting a new line of treatment was 75.9% (n = 108; median 8 CTCs/7.5 ml blood) at a cut off of ≥2 CTCs. PFS was inferior for mBC patients with baseline CTC count ≥5 CTCs/7.5 ml blood vs. those with < 5 CTCs/7.5 ml blood (median PFS 4.3 vs. 7.0 months; p-value 0.037). The prognostic relevance of CTCs was most significant in patients with HER2- mBC (median PFS 4.1 vs. 8.3 months; p-value 0.032), luminal (HR+HER2-) subtype (median PFS 4.2 vs. 8.3 months; p-value 0.048), and patients who had one or more prior treatments (median PFS 4.2 vs. 7.0 months; p-value 0.02). On multivariable analysis, baseline CTC level (hazard ratio (HR) 1.84, p-value 0.02) and pre-treatment status (HR 1.87, p-value 0.05) were independent predictors of PFS.CONCLUSIONS:
This work demonstrates the prognostic significance of CTCs in mBC detected using a label-free size-based enrichment platform.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Células Neoplásicas Circulantes
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Middle aged
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article