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EPA and DHA have selective toxicity for PBMCs from multiple myeloma patients in a partly caspase-dependent manner.
Mortaz, Esmaeil; Moloudizargari, Milad; Khosravi, Adnan; Asghari, Mohammad Hossein; Movassaghi, Mehrnaz; Varahram, Mohammad; Vaezi, Mohammad; Redegeld, Frank A; Garssen, Johan.
Afiliação
  • Mortaz E; Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht Universit
  • Moloudizargari M; Department of Immunology, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Khosravi A; Chronic Respiratory Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Asghari MH; Department of Pharmacology, School of Medicine, Babol University of Medical Sciences, Babol, Iran; Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
  • Movassaghi M; Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Varahram M; Mycobacteriology Research Center (MRC) National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Vaezi M; Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran; Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Tehran, Iran.
  • Redegeld FA; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, the Netherlands. Electronic address: F.A.M.Redegeld@uu.nl.
  • Garssen J; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, the Netherlands; Nutricia Research Centre for Specialized Nutrition, Utrecht, Netherlands.
Clin Nutr ; 39(7): 2137-2143, 2020 07.
Article em En | MEDLINE | ID: mdl-31558292
Poly-unsaturated fatty acids (PUFAs) have been shown to have cytotoxic effects in both solid and non-solid tumors. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are among the most studied PUFAs. The aim of the present study was to evaluate the cytotoxic effects of these two fatty acids (FAs) in the peripheral blood mononuclear cells (PBMCs) obtained from untreated patients (new cases) with confirmed symptomatic multiple myeloma (MM). Our results showed that EPA at the concentration of 100 µM and DHA at 50 and 100 µM induce potent apoptotic effects in the PBMCs of MM patients (P < 0.05) as evidenced by Annexin V and propidium iodide (PI) staining, while they have little or no effects on the PBMCs isolated from healthy donors (P > 0.05). The observed effects were concentration- and time-dependent and 72 h treatment with DHA at a concentration of 100 µM had the strongest effect (P < 0.01). CD138 + cells isolated from MM patients showed great sensitivity to EPA/DHA. EPA- and DHA-induced apoptosis was significantly inhibited by the pan-caspase inhibitor (Z-VAD-FMK), indicating that cell death was at least partly dependent on caspase activation. The results of the present study showed that EPA and DHA have selective toxicities for malignant human plasma cells from MM patients, but not for mononuclear cells of healthy donors. These results warrant further studies with larger study populations to investigate the usefulness of PUFAs as a promising adjunctive therapy in the treatment of MM.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Plasmócitos / Leucócitos Mononucleares / Ácido Eicosapentaenoico / Ácidos Docosa-Hexaenoicos / Apoptose / Caspases / Mieloma Múltiplo / Antineoplásicos Tipo de estudo: Observational_studies Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Plasmócitos / Leucócitos Mononucleares / Ácido Eicosapentaenoico / Ácidos Docosa-Hexaenoicos / Apoptose / Caspases / Mieloma Múltiplo / Antineoplásicos Tipo de estudo: Observational_studies Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article