Therapeutic effects of bone mesenchymal stem cells on oral and maxillofacial defects: a novel signaling pathway involving miR-31/CXCR4/Akt axis.
J Recept Signal Transduct Res
; 39(4): 321-330, 2019 Aug.
Article
em En
| MEDLINE
| ID: mdl-31573375
ABSTRACT
Context Although bone mesenchymal stem cells (BMSCs) have been used for the treatment of oral and maxillofacial defects, the survival rate and limited proliferation reduces the therapeutic efficiency of BMSC.Objective:
The aim of our study is to explore the role of miR-31 in regulating survival, proliferation, and migration of BMSC in vitro.Materials andmethods:
LPS was used in vitro to induce BMSC damage and then miR-31 was used to incubate with BMSC. Subsequently, BMSC proliferation, survival, and migration were determined via ELISA, qPCR, western blots, and immunofluorescence.Results:
The expression of miR-31 was downregulated in response to LPS stress. Interestingly, supplementation of miR-31 could reverse the survival, proliferation and migration of BMSC under LPS. Mechanically, miR-31 treatment inhibited the activation of caspase, and thus promoted BMSC survival. Besides, miR-31 upregulated the genes related to cell proliferation, an effect that was followed by an increase in the levels of migratory factors. Further, we found that miR-31 treatment activated the CXCR4/Akt pathway and blockade of CXCR4/Akt could abolish the beneficial effects of miR-31 on BMSC proliferation, survival, and migration.Conclusions:
miR-31 could increase the therapeutic efficiency of BMSC via the CXCR4/Akt pathway.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Receptores CXCR4
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MicroRNAs
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Transplante de Células-Tronco Mesenquimais
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Proteínas Proto-Oncogênicas c-akt
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Células-Tronco Mesenquimais
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Traumatismos Maxilofaciais
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Doenças da Boca
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article