Your browser doesn't support javascript.
loading
Glycan-mediated enhancement of reovirus receptor binding.
Koehler, Melanie; Aravamudhan, Pavithra; Guzman-Cardozo, Camila; Dumitru, Andra C; Yang, Jinsung; Gargiulo, Serena; Soumillion, Patrice; Dermody, Terence S; Alsteens, David.
Afiliação
  • Koehler M; Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
  • Aravamudhan P; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
  • Guzman-Cardozo C; Center for Microbial Pathogenesis, UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA, USA.
  • Dumitru AC; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
  • Yang J; Center for Microbial Pathogenesis, UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA, USA.
  • Gargiulo S; Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
  • Soumillion P; Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
  • Dermody TS; Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
  • Alsteens D; Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
Nat Commun ; 10(1): 4460, 2019 10 01.
Article em En | MEDLINE | ID: mdl-31575869
ABSTRACT
Viral infection is an intricate process that requires the concerted action of both viral and host cell components. Entry of viruses into cells is initiated by interactions between viral proteins and their cell surface receptors. Despite recent progress, the molecular mechanisms underlying the multistep reovirus entry process are poorly understood. Using atomic force microscopy, we investigated how the reovirus σ1 attachment protein binds to both α-linked sialic acid (α-SA) and JAM-A cell-surface receptors. We discovered that initial σ1 binding to α-SA favors a strong multivalent anchorage to JAM-A. The enhanced JAM-A binding by virions following α-SA engagement is comparable to JAM-A binding by infectious subvirion particles (ISVPs) in the absence of α-SA. Since ISVPs have an extended σ1 conformer, this finding suggests that α-SA binding triggers a conformational change in σ1. These results provide new insights into the function of viral attachment proteins in the initiation of infection and open new avenues for the use of reoviruses as oncolytic agents.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Ligação Proteica / Receptores Virais / Reoviridae / Proteínas Virais / Ligação Viral Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Ligação Proteica / Receptores Virais / Reoviridae / Proteínas Virais / Ligação Viral Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article