X-chromosome upregulation is driven by increased burst frequency.
Nat Struct Mol Biol
; 26(10): 963-969, 2019 10.
Article
em En
| MEDLINE
| ID: mdl-31582851
ABSTRACT
Ohno's hypothesis postulates that upregulation of X-linked genes rectifies their dosage imbalance relative to autosomal genes, which are present in two active copies per cell. Here we have dissected X-chromosome upregulation into the kinetics of transcription, inferred from allele-specific single-cell RNA sequencing data from somatic and embryonic mouse cells. We confirmed increased X-chromosome expression levels in female and male cells and found that the X chromosome achieved upregulation by elevated burst frequencies. By monitoring transcriptional kinetics in differentiating female mouse embryonic stem cells, we found that increased burst frequency was established on the active X chromosome when X inactivation took place on the other allele. Thus, our study provides mechanistic insights into X-chromosome upregulation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromossomo X
/
Ativação Transcricional
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Regulação para Cima
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article