Cell confinement reveals a branched-actin independent circuit for neutrophil polarity.
PLoS Biol
; 17(10): e3000457, 2019 10.
Article
em En
| MEDLINE
| ID: mdl-31600188
ABSTRACT
Migratory cells use distinct motility modes to navigate different microenvironments, but it is unclear whether these modes rely on the same core set of polarity components. To investigate this, we disrupted actin-related protein 2/3 (Arp2/3) and the WASP-family verprolin homologous protein (WAVE) complex, which assemble branched actin networks that are essential for neutrophil polarity and motility in standard adherent conditions. Surprisingly, confinement rescues polarity and movement of neutrophils lacking these components, revealing a processive bleb-based protrusion program that is mechanistically distinct from the branched actin-based protrusion program but shares some of the same core components and underlying molecular logic. We further find that the restriction of protrusion growth to one site does not always respond to membrane tension directly, as previously thought, but may rely on closely linked properties such as local membrane curvature. Our work reveals a hidden circuit for neutrophil polarity and indicates that cells have distinct molecular mechanisms for polarization that dominate in different microenvironments.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Quimiotaxia
/
Actinas
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Polaridade Celular
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Complexo 2-3 de Proteínas Relacionadas à Actina
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Família de Proteínas da Síndrome de Wiskott-Aldrich
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article