Antiproliferative and apoptotic effects of the natural alkaline water (Zamzam) in breast cancer cell line MCF-7.
J Cancer Res Ther
; 15(5): 1098-1104, 2019.
Article
em En
| MEDLINE
| ID: mdl-31603117
ABSTRACT
BACKGROUND:
Zamzam water (ZW) is a natural alkaline water that contains several minerals that may represent a powerful tool for cancer therapy.OBJECTIVES:
In this research, in vitro antiproliferative and apoptotic effects of ZW were investigated in the human breast cancer cell line MCF-7. MATERIALS ANDMETHODS:
This study was conducted between January 2015 and February 2016. The effects of ZW on the morphology and the cell viability of human breast cancer cell line MCF-7 were determined. The cell death type and cell cycle changes were investigated using flow cytometry. Finally, reactive oxygen species (ROS) were also measured by fluorometric technique.RESULTS:
MCF-7 cells treated with either ZW with adjusted pH at 7.2 or unadjusted pH at 8 showed reduced cell viability of cancerous cells. The cell death occurred through the apoptosis pathway under both treatment conditions. The treated MCF-7 cells were arrested in the G2/M phase and decreased in the G1 phase. Only the unadjusted pH ZW sample demonstrated an increase in the production of both cytoplasmic and mitochondrial ROS in MCF-7 cells.CONCLUSION:
All the results in the present study indicated, for the first time, that ZW might have anticancer and apoptotic effects on breast cancer cell line.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Água
/
Apoptose
/
Proliferação de Células
/
Antineoplásicos
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article