Comparison of a medium supplement--'Nu-Serum'--and foetal calf serum in RPMI 1640 used for the murine allogeneic mixed lymphocyte reaction.

ABSTRACT

A commercially available medium supplement, Nu-Serum, was compared with foetal calf serum for its ability to support the murine allogeneic mixed lymphocyte reaction. Nu-Serum supported proliferation whilst at the same time producing significantly lower counts in unstimulated cultures. There was considerably less batch-to-batch variation with Nu-Serum than with foetal calf serum, which obviated the need for pretesting in order to find a suitable batch.