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mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM.
Fu, Zhifei; Peng, Dingming; Zhang, Mingshu; Xue, Fudong; Zhang, Rui; He, Wenting; Xu, Tao; Xu, Pingyong.
Afiliação
  • Fu Z; Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
  • Peng D; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
  • Zhang M; Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
  • Xue F; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
  • Zhang R; Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China. zhangmingshu@ibp.ac.cn.
  • He W; Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
  • Xu T; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
  • Xu P; Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Nat Methods ; 17(1): 55-58, 2020 01.
Article em En | MEDLINE | ID: mdl-31611693
ABSTRACT
Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO4) treatment for improved SR-CLEM.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tetróxido de Ósmio / Manejo de Espécimes / Microscopia Eletrônica / Organelas / Resinas Epóxi / Proteínas Luminescentes Limite: Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
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XML
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tetróxido de Ósmio / Manejo de Espécimes / Microscopia Eletrônica / Organelas / Resinas Epóxi / Proteínas Luminescentes Limite: Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article