Synthetic Chemical Probes That Dissect Vitamin D Activities.
ACS Chem Biol
; 14(12): 2851-2858, 2019 12 20.
Article
em En
| MEDLINE
| ID: mdl-31618573
ABSTRACT
Vitamin D3 metabolites are capable of controlling gene expression in mammalian cells through two independent pathways vitamin D receptor (VDR) and sterol regulatory element-binding protein (SREBP) pathways. In the present study, we dissect the complex biological activity of vitamin D by designing synthetic vitamin D3 analogs specific for VDR or SREBP pathway, i.e., a VDR activator that lacks SREBP inhibitory activity, or an SREBP inhibitor devoid of VDR activity. These synthetic vitamin D probes permitted identification of one of the vitamin D-responsive genes, Soat1, as an SREBP-suppressed gene. The chemical probes developed in the present study may prove useful in dissecting the intricate interplay of vitamin D actions, thereby providing insights into how vitamin D target genes are regulated.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vitamina D
/
Sondas Moleculares
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article