Occurrence of antibiotics and their associations with antibiotic resistance genes and bacterial communities in Guangdong coastal areas.

ABSTRACT

The concentration of 8 antibiotics and 21 antibiotic resistance genes were investigated in the coastal areas of Guangdong, China. Total concentrations of antibiotics ranged from 0.43 ng/L to 1040.31 ng/L. The concentrations of tetracyclines were much higher than that of sulfonamides in most sampling sites. The abundance of target antibiotic resistance genes ranged from 1.82 × 105 to 5.9 × 109 copies/mL and tetM accounted for the highest percentages of detected antibiotic resistance genes in most sampling sites. Furthermore, the dominant phyla in water samples were Proteobacteria, Bacteroidetes and Actinobacteria. The relationship between antibiotics, antibiotic resistance genes, and bacterial communities was also investigated. As a result, the abundance of sul1 was positively correlated with the concentration of sulfadiazine, sulfamethoxazole and sulfonamide p-methyl oxypyrimidine. Besides, sulfonamide p-methyl oxypyrimidine, sulfadiazine and p-aminobenzenesulfonamide were significantly correlated with the bacterial communities. These findings suggested that the residues of antibiotics in coastal areas of Guangdong affect the distribution of antibiotic resistance genes and alter the microbial communities.