Multimeric Ionotropic Purinoceptor Detection by Protein Cross-Linking.
Methods Mol Biol
; 2041: 147-153, 2020.
Article
em En
| MEDLINE
| ID: mdl-31646486
ABSTRACT
P2X receptor subunits (P2X1 to P2X7) assemble to form trimeric homomers or heteromers. Here, we describe the use of protein cross-linking to study the composition of P2X receptor complexes. This simple protocol is useful for determining the stoichiometry of P2X heteromeric receptors as well as for assessing the effect of point mutation, truncation, or concatenation on the quaternary architecture of these receptors.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Receptores Purinérgicos
/
Reagentes de Ligações Cruzadas
/
Multimerização Proteica
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article