Time-resolved Fluorescence and Generalized Polarization: Innovative tools to assess bull sperm membrane dynamics during slow freezing.
Cryobiology
; 91: 69-76, 2019 12.
Article
em En
| MEDLINE
| ID: mdl-31678178
ABSTRACT
During slow freezing, spermatozoa undergo membrane alterations that compromise their ability of fertilizing. These alterations are cause either by cold shock or by the use of cryoprotectants known to be cytotoxic. However, little is known about the membrane changes that occurred during freezing. Here, we combined Generalized Polarization (GP), Time-resolved Fluorescence and laurdan fluorescence properties to investigate the changes in membrane fluidity and dynamics during slow freezing of bull sperm. We successfully demonstrated that laurdan may be distributed in three different local environments that correspond to different membrane lipid composition. These environments wont behave the same way when the cells will be subjected to either a chemical treatment (adding the cryoprotectants) or a physical treatment (freezing).
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
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Criopreservação
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Membrana Celular
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Lauratos
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Fluidez de Membrana
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2-Naftilamina
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article