Fast isolation of highly specific population of platelet-derived extracellular vesicles from blood plasma by affinity monolithic column, immobilized with anti-human CD61 antibody.

Multia, Evgen; Tear, Crystal Jing Ying; Palviainen, Mari; Siljander, Pia; Riekkola, Marja-Liisa

Multia, Evgen; Tear, Crystal Jing Ying; Palviainen, Mari; Siljander, Pia; Riekkola, Marja-Liisa.

Afiliação

Multia E; Department of Chemistry, P.O. Box 55, FI-00014, University of Helsinki, Finland.
Tear CJY; Department of Chemistry, P.O. Box 55, FI-00014, University of Helsinki, Finland.
Palviainen M; EV-group, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Finland.
Siljander P; EV-group, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Finland.
Riekkola ML; Department of Chemistry, P.O. Box 55, FI-00014, University of Helsinki, Finland. Electronic address: marja-liisa.riekkola@helsinki.fi.

Anal Chim Acta ; 1091: 160-168, 2019 Dec 24.

Article em En | MEDLINE | ID: mdl-31679569

Assuntos

Plaquetas/citologia; Cromatografia de Afinidade/métodos; Vesículas Extracelulares; Animais; Anticorpos Imobilizados/imunologia; Difusão Dinâmica da Luz; Vesículas Extracelulares/química; Vesículas Extracelulares/imunologia; Fracionamento por Campo e Fluxo; Humanos; Integrina beta3/imunologia; Camundongos; Tamanho da Partícula; Ácidos Polimetacrílicos/química

Palavras-chave

CD61; Extracellular vesicles; Immunoaffinity chromatography; Isolation; Monolithic disk column; Platelet-derived vesicles

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Plaquetas / Cromatografia de Afinidade / Vesículas Extracelulares Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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