Method for growing primitive erythroid progenitors (BFU-E) from rat bone marrow.
Exp Hematol
; 16(9): 790-3, 1988 Oct.
Article
em En
| MEDLINE
| ID: mdl-3169160
ABSTRACT
A method for growing erythroid bursts from rat bone marrow cells is presented. The methylcellulose culture technique with rat spleen-conditioned medium was used. Successful growth of rat BFU-E was obtained with 20% fetal bovine serum when anemic rat plasma was used as the source of erythropoietin. Plating efficiency was 60 BFU-E per 2 x 10(5) nucleated cells seeded. The proliferative activity determined by cytosine arabinoside (Ara-C) suicide was about 22%. The effect of chronic hypertransfusion on erythroid colony formation is presented.
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Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Células-Tronco Hematopoéticas
/
Eritroblastos
/
Ensaio de Unidades Formadoras de Colônias
Limite:
Animals
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article