An In Vitro Method to Analyze Glucose Uptake in Podocytes.
Methods Mol Biol
; 2067: 139-143, 2020.
Article
em En
| MEDLINE
| ID: mdl-31701450
ABSTRACT
Podocytes are terminally differentiated, insulin-sensitive cells of the glomerular filtration barrier, with a central role in filtration barrier maintenance. Podocyte injury is one of the earliest features observed in diabetic nephropathy (DN) and plays a key role in the development of albuminuria. Several factors are associated with diabetes-mediated podocyte injury, including dysregulated metabolic pathways and insulin sensitivity (Lay & Coward, Nephrol Dial Transplant 291127-1133, 2014); thus, reliable assays to study these responses are key in understanding podocyte alterations in DN. Here, we detail an in vitro method to analyze glucose uptake in conditionally immortalized human podocytes (Saleem, J Am Soc Nephrol 13630-638, 2002; Coward, Diabetes 543095-3102, 2005); this assay is useful for detecting changes in podocyte metabolism, nutrient sensing, and insulin sensitivity.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Podócitos
/
Glucose
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article