Total saponins of Bolbostemma paniculatum (maxim.) Franquet exert antitumor activity against MDA-MB-231 human breast cancer cells via inhibiting PI3K/Akt/mTOR pathway.
BMC Complement Altern Med
; 19(1): 304, 2019 Nov 08.
Article
em En
| MEDLINE
| ID: mdl-31703679
ABSTRACT
BACKGROUND:
The aim of the present study was to examine the effects of the Bolbostemma paniculatum (Maxim.) Franquet (BP) active compound, BP total saponins (BPTS), on MDA-MB-231 cells, and investigate the underlying mechanism regarding BPTS-mediated attenuation of the PI3K/Akt/mTOR pathway.METHODS:
The effect of BPTS on cytotoxicity, induction of apoptosis and migration on MDA-MB-231 cells at three different concentrations was investigated. A CCK-8 assay, wound-healing assay and flow cytometry were used to demonstrate the effects of BPTS. Additionally, expression of the primary members of the PI3K/Akt/mTOR signaling pathway was assessed using western blotting. To verify the underlying mechanisms, a PI3K inhibitor and an mTOR inhibitor were used.RESULTS:
BPTS inhibited proliferation of MDA-MB-231 cells with an IC50 value of 10 µg/mL at 48 h. BPTS inhibited migration of MDA-MB-231 cells, and the western blot results demonstrated that BPTS reduced p-PI3K, p-Akt and p-mTOR protein expression levels in MDA-MB-231 cells. Additionally, the results were confirmed using a PI3K inhibitor and an mTOR inhibitor. BPTS decreased proliferation and migration of MDA-MB-231 cells possibly through inhibiting the PI3K/Akt/mTOR signaling pathway.CONCLUSIONS:
The results highlight the therapeutic potential of BPTS for treating patients with triple-negative breast cancer.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Saponinas
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Neoplasias da Mama
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Extratos Vegetais
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Cucurbitaceae
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Fosfatidilinositol 3-Quinases
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Proteínas Proto-Oncogênicas c-akt
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Serina-Treonina Quinases TOR
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Antineoplásicos Fitogênicos
Limite:
Female
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Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article