The cyclin B2/CDK1 complex inhibits separase activity in mouse oocyte meiosis I.
Development
; 146(23)2019 12 02.
Article
em En
| MEDLINE
| ID: mdl-31704793
ABSTRACT
Chromosome segregation is driven by separase, activity of which is inhibited by binding to securin and cyclin B1/CDK1. In meiosis, premature separase activity will induce aneuploidy or abolish chromosome segregation owing to the untimely destruction of cohesin. Recently, we have proved that cyclin B2 can compensate for cyclin B1 in CDK1 activation for the oocyte meiosis G2/M transition. In the present study, we identify an interaction between cyclin B2/CDK1 and separase in mouse oocytes. We find that cyclin B2 degradation is required for separase activation during the metaphase I-anaphase I transition because the presence of stable cyclin B2 leads to failure of homologous chromosome separation and to metaphase I arrest, especially in the simultaneous absence of securin and cyclin B1. Moreover, non-phosphorylatable separase rescues the separation of homologous chromosomes in stable cyclin B2-arrested cyclin B1-null oocytes. Our results indicate that cyclin B2/CDK1 is also responsible for separase inhibition via inhibitory phosphorylation to regulate chromosome separation in oocyte meiosis, which may not occur in other cell types.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
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Proteína Quinase CDC2
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Segregação de Cromossomos
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Ciclina B2
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Separase
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Anáfase
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Metáfase
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article