Improved Electrophoretic Separation to Assist the Monitoring of Bcl-xL Post-Translational Modifications.
Int J Mol Sci
; 20(22)2019 Nov 08.
Article
em En
| MEDLINE
| ID: mdl-31717257
ABSTRACT
Bcl-xL is an oncogene of which the survival functions are finely tuned by post-translational modifications (PTM). Within the Bcl-2 family of proteins, Bcl-xL shows unique eligibility to deamidation, a time-related spontaneous reaction. Deamidation is still a largely overlooked PTM due to a lack of easy techniques to monitor AsnâAsp/IsoAsp conversions or GluâGln conversions. Being able to detect PTMs is essential to achieve a comprehensive description of all the regulatory mechanisms and functions a protein can carry out. Here, we report a gel composition improving the electrophoretic separation of deamidated forms of Bcl-xL generated either by mutagenesis or by alkaline treatment. Importantly, this new gel formulation proved efficient to provide the long-sought evidence that even doubly-deamidated Bcl-xL remains eligible for regulation by phosphorylation.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Processamento de Proteína Pós-Traducional
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Eletroforese
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Proteína bcl-X
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article