Continual Conscious Bioluminescent Imaging in Freely Moving Mice.
Methods Mol Biol
; 2081: 161-175, 2020.
Article
em En
| MEDLINE
| ID: mdl-31721124
ABSTRACT
In vivo bioluminescent imaging allows the detection of reporter gene expression in rodents in real time. Here we describe a novel technology whereby we can generate somatotransgenic rodents with the use of a viral vector carrying a luciferase transgene. We are able to achieve long term luciferase expression by a single injection of lentiviral or adeno-associated virus vectors to newborn mice. Further, we describe whole body bioluminescence imaging of conscious mice in a noninvasive manner, thus enforcing the 3R's (replacement, reduction, and refinement) of biomedical animal research.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Genes Reporter
/
Medições Luminescentes
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article