Substantially improving the enantioconvergence of PvEH1, a Phaseolus vulgaris epoxide hydrolase, towards m-chlorostyrene oxide by laboratory evolution.

Zong, Xun-Cheng; Li, Chuang; Xu, Yao-Hui; Hu, Die; Hu, Bo-Chun; Zang, Jia; Wu, Min-Chen

Zong, Xun-Cheng; Li, Chuang; Xu, Yao-Hui; Hu, Die; Hu, Bo-Chun; Zang, Jia; Wu, Min-Chen.

Afiliação

Zong XC; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Li C; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Xu YH; The Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University, Wuxi, 214002, China.
Hu D; Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China.
Hu BC; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Zang J; The Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University, Wuxi, 214002, China. zangj1976@sina.com.
Wu MC; Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China. biowmc@126.com.

Microb Cell Fact ; 18(1): 202, 2019 Nov 18.

Article em En | MEDLINE | ID: mdl-31739786

Assuntos

Evolução Molecular Direcionada; Epóxido Hidrolases/genética; Phaseolus/enzimologia; Escherichia coli; Genes de Plantas; Modelos Moleculares; Mutagênese Sítio-Dirigida; Phaseolus/genética; Transformação Bacteriana

Palavras-chave

Enantioconvergence; Epoxide hydrolase; Laboratory evolution; Substrate-binding pocket; m-Chlorophenyl-1,2-ethanediol; m-Chlorostyrene oxide

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Evolução Molecular Direcionada / Phaseolus / Epóxido Hidrolases Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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